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Fig. 6. Effects of AWE on Pb induced hepatic inflammation in mice (A-C) Liver relative mRNA expression of IL-1β (A), IL-6 (B), and TNF-α (C) was quantified by qPCR in each group. mRNA levels of target genes were normalized to GAPDH. (D–F) Serum protein levels of IL-1β (D), IL-6 (E), and TNF-α (F) were determined in each group. (G) Representative Western blot images of <t>NLRP3,</t> <t>Caspase-</t> 1, <t>GSDMD,</t> and GAPDH of the liver sample from each group. (H–J) Quantitative analysis of NLRP3 (H), Caspase-1 (I), and GSDMD (J). GAPDH is used as a loading control for protein normalization. The data are expressed as mean ± SD. n = (3–6) per group. **P < 0.01 compared with the blank group; #P < 0.05, ##P < 0.01 compared with the Pb group. $P < 0.05, $$P < 0.01 compared with the Pb + AWE (0.8) group.
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Fig. 6. Effects of AWE on Pb induced hepatic inflammation in mice (A-C) Liver relative mRNA expression of IL-1β (A), IL-6 (B), and TNF-α (C) was quantified by qPCR in each group. mRNA levels of target genes were normalized to GAPDH. (D–F) Serum protein levels of IL-1β (D), IL-6 (E), and TNF-α (F) were determined in each group. (G) Representative Western blot images of <t>NLRP3,</t> <t>Caspase-</t> 1, <t>GSDMD,</t> and GAPDH of the liver sample from each group. (H–J) Quantitative analysis of NLRP3 (H), Caspase-1 (I), and GSDMD (J). GAPDH is used as a loading control for protein normalization. The data are expressed as mean ± SD. n = (3–6) per group. **P < 0.01 compared with the blank group; #P < 0.05, ##P < 0.01 compared with the Pb group. $P < 0.05, $$P < 0.01 compared with the Pb + AWE (0.8) group.
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Fig. 6. Effects of AWE on Pb induced hepatic inflammation in mice (A-C) Liver relative mRNA expression of IL-1β (A), IL-6 (B), and TNF-α (C) was quantified by qPCR in each group. mRNA levels of target genes were normalized to GAPDH. (D–F) Serum protein levels of IL-1β (D), IL-6 (E), and TNF-α (F) were determined in each group. (G) Representative Western blot images of <t>NLRP3,</t> <t>Caspase-</t> 1, <t>GSDMD,</t> and GAPDH of the liver sample from each group. (H–J) Quantitative analysis of NLRP3 (H), Caspase-1 (I), and GSDMD (J). GAPDH is used as a loading control for protein normalization. The data are expressed as mean ± SD. n = (3–6) per group. **P < 0.01 compared with the blank group; #P < 0.05, ##P < 0.01 compared with the Pb group. $P < 0.05, $$P < 0.01 compared with the Pb + AWE (0.8) group.
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Fig. 6. Effects of AWE on Pb induced hepatic inflammation in mice (A-C) Liver relative mRNA expression of IL-1β (A), IL-6 (B), and TNF-α (C) was quantified by qPCR in each group. mRNA levels of target genes were normalized to GAPDH. (D–F) Serum protein levels of IL-1β (D), IL-6 (E), and TNF-α (F) were determined in each group. (G) Representative Western blot images of <t>NLRP3,</t> <t>Caspase-</t> 1, <t>GSDMD,</t> and GAPDH of the liver sample from each group. (H–J) Quantitative analysis of NLRP3 (H), Caspase-1 (I), and GSDMD (J). GAPDH is used as a loading control for protein normalization. The data are expressed as mean ± SD. n = (3–6) per group. **P < 0.01 compared with the blank group; #P < 0.05, ##P < 0.01 compared with the Pb group. $P < 0.05, $$P < 0.01 compared with the Pb + AWE (0.8) group.
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Fig. 6. Effects of AWE on Pb induced hepatic inflammation in mice (A-C) Liver relative mRNA expression of IL-1β (A), IL-6 (B), and TNF-α (C) was quantified by qPCR in each group. mRNA levels of target genes were normalized to GAPDH. (D–F) Serum protein levels of IL-1β (D), IL-6 (E), and TNF-α (F) were determined in each group. (G) Representative Western blot images of NLRP3, Caspase- 1, GSDMD, and GAPDH of the liver sample from each group. (H–J) Quantitative analysis of NLRP3 (H), Caspase-1 (I), and GSDMD (J). GAPDH is used as a loading control for protein normalization. The data are expressed as mean ± SD. n = (3–6) per group. **P < 0.01 compared with the blank group; #P < 0.05, ##P < 0.01 compared with the Pb group. $P < 0.05, $$P < 0.01 compared with the Pb + AWE (0.8) group.

Journal: Journal of ethnopharmacology

Article Title: Artichoke water extract protects against Lead-induced hepatotoxicity by activating Nrf2 signaling and inhibiting NLRP3/caspase-1/GSDMD-mediated pyroptosis.

doi: 10.1016/j.jep.2025.119654

Figure Lengend Snippet: Fig. 6. Effects of AWE on Pb induced hepatic inflammation in mice (A-C) Liver relative mRNA expression of IL-1β (A), IL-6 (B), and TNF-α (C) was quantified by qPCR in each group. mRNA levels of target genes were normalized to GAPDH. (D–F) Serum protein levels of IL-1β (D), IL-6 (E), and TNF-α (F) were determined in each group. (G) Representative Western blot images of NLRP3, Caspase- 1, GSDMD, and GAPDH of the liver sample from each group. (H–J) Quantitative analysis of NLRP3 (H), Caspase-1 (I), and GSDMD (J). GAPDH is used as a loading control for protein normalization. The data are expressed as mean ± SD. n = (3–6) per group. **P < 0.01 compared with the blank group; #P < 0.05, ##P < 0.01 compared with the Pb group. $P < 0.05, $$P < 0.01 compared with the Pb + AWE (0.8) group.

Article Snippet: Primary antibodies against GSDMD (39754), Caspase-1 (83383), and GAPDH (2118) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Control